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double antibody sandwich elisa kit  (Elabscience Biotechnology)


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    Elabscience Biotechnology double antibody sandwich elisa kit
    AMBP promotes imbalance of BCAA metabolism. (A) mRNA transcripts of AMBP in patients with different grades of IVDD and relative quantitative analysis. (B) mRNA transcripts of AMBP in TNF-α-stimulated HNPC cells and relative quantitative analysis. (C) Detection of inhibition efficiency of small interfering RNAs against different targets. (D) Serum concentrations of AMBP in normal, T2DM, IVDD, and T2DM combined with IVDD patients were examined using <t>ELISA.</t> (E) Representative β-gal staining images of HNPC cells before and after AMBP inhibition. (F) Correlation analysis between AMBP expression and β-gal expression. (G) mRNA transcripts of BCAA metabolism-related enzymes in TNF-α-stimulated HNPC cells before and after AMBP inhibition and relative quantitative analysis.
    Double Antibody Sandwich Elisa Kit, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sandwich+elisa+kit/Human+AMBP+(Alpha-1-Microglobulin%2FBikunin+Precursor)+ELISA+Kit/pmc13124742-121-7-11
    Average 94 stars, based on 1 article reviews
    double antibody sandwich elisa kit - by Bioz Stars, 2026-09
    94/100 stars

    Images

    1) Product Images from "Screening of metabolic-related biomarkers linking intervertebral disc degeneration and type 2 diabetes based on comprehensive bioinformatics analysis and machine learning"

    Article Title: Screening of metabolic-related biomarkers linking intervertebral disc degeneration and type 2 diabetes based on comprehensive bioinformatics analysis and machine learning

    Journal: Biochemistry and Biophysics Reports

    doi: 10.1016/j.bbrep.2026.102593

    AMBP promotes imbalance of BCAA metabolism. (A) mRNA transcripts of AMBP in patients with different grades of IVDD and relative quantitative analysis. (B) mRNA transcripts of AMBP in TNF-α-stimulated HNPC cells and relative quantitative analysis. (C) Detection of inhibition efficiency of small interfering RNAs against different targets. (D) Serum concentrations of AMBP in normal, T2DM, IVDD, and T2DM combined with IVDD patients were examined using ELISA. (E) Representative β-gal staining images of HNPC cells before and after AMBP inhibition. (F) Correlation analysis between AMBP expression and β-gal expression. (G) mRNA transcripts of BCAA metabolism-related enzymes in TNF-α-stimulated HNPC cells before and after AMBP inhibition and relative quantitative analysis.
    Figure Legend Snippet: AMBP promotes imbalance of BCAA metabolism. (A) mRNA transcripts of AMBP in patients with different grades of IVDD and relative quantitative analysis. (B) mRNA transcripts of AMBP in TNF-α-stimulated HNPC cells and relative quantitative analysis. (C) Detection of inhibition efficiency of small interfering RNAs against different targets. (D) Serum concentrations of AMBP in normal, T2DM, IVDD, and T2DM combined with IVDD patients were examined using ELISA. (E) Representative β-gal staining images of HNPC cells before and after AMBP inhibition. (F) Correlation analysis between AMBP expression and β-gal expression. (G) mRNA transcripts of BCAA metabolism-related enzymes in TNF-α-stimulated HNPC cells before and after AMBP inhibition and relative quantitative analysis.

    Techniques Used: Inhibition, Enzyme-linked Immunosorbent Assay, Staining, Expressing

    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: The Effects of Interval Resistance-Aerobic Training and Fisetin Supplementation on Asprosin and Selected Adipokines in Obese Men: A Double-Blind Randomized Control Trial.
    Article Snippet: .. Serum concentrations were analyzed using specific human enzyme-linked immunosorbent assay (ELISA) kits, among which Asprosin, MCP-1, and adiponectin were designated as primary outcomes; specifically, Asprosin concentration was measured with a sandwich ELISA kit (Elabscience Biotechnology, Wuhan, China; Catalog No. E-EL-H0515; detection range 0.156–10 ng/mL; sensitivity 0.094 ng/mL; Intra-assay CV < 10% and Inter-assay CV < 10%), MCP-1 concentration with a sandwich ELISA kit (R&D Systems, Minneapolis, MN, USA; Catalog No. DCP00; sensitivity 10 pg/mL; Intra-CV = 7.8% and Inter-CV = 6.7%), and adiponectin concentration with an ELISA kit (Biovendor, Brno, Czech Republic; Catalog No. RD195023100; sensitivity 26 ng/mL; Intra-CV = 4.9% and Inter-CV = 6.7%). .. Furthermore, the lipid profile, anthropometric indices, and other markers were considered as secondary variables to adopt a structured approach in addressing multiplicity; in this regard, total cholesterol (TC) and triglycerides (TGs) were measured using enzymatic methods (CHODPAP), while HDL-C and LDL-C were measured via photometric methods (quantitative diagnostic kit by Pars Azmoon, Tehran, Iran; with a coefficient of 1.8% and sensitivity of 1 mg/dL for HDL, and a coefficient of 1.2% and sensitivity of 1 mg/dL for LDL), and leptin concentration was also determined with an ELISA kit (Biovendor, Czech Republic; Catalog No. RD191001100; sensitivity 0.2 ng/mL; Intra-CV = 5.9% and Inter-CV = 5.6%).

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    Article Title: The Effects of Interval Resistance—Aerobic Training and Fisetin Supplementation on Asprosin and Selected Adipokines in Obese Men: A Double-Blind Randomized Control Trial
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    Concentration Assay:

    Article Title: The Effects of Interval Resistance-Aerobic Training and Fisetin Supplementation on Asprosin and Selected Adipokines in Obese Men: A Double-Blind Randomized Control Trial.
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    Article Title: Serum Perilipin-2 as a Novel Biomarker for Obstructive Sleep Apnea: Association with Hypoxic Burden and Disease Severity.
    Article Snippet: .. Assessment of Serum Perilipin-2 (PLIN 2) Concentration Serum PLIN-2 concentrations were determined using a commercially available sandwich ELISA kit (Human ADRP/Perilipin-2, Cat No: E-EL-H0278, Elabscience, Houston, TX, USA) according to the manufacturer’s instructions. ..

    Article Title: The Effects of Interval Resistance—Aerobic Training and Fisetin Supplementation on Asprosin and Selected Adipokines in Obese Men: A Double-Blind Randomized Control Trial
    Article Snippet: .. Serum concentrations were analyzed using specific human enzyme-linked immunosorbent assay (ELISA) kits, among which Asprosin, MCP-1, and adiponectin were designated as primary outcomes; specifically, Asprosin concentration was measured with a sandwich ELISA kit (Elabscience Biotechnology, Wuhan, China; Catalog No. E-EL-H0515; detection range 0.156–10 ng/mL; sensitivity 0.094 ng/mL; Intra-assay CV < 10% and Inter-assay CV < 10%), MCP-1 concentration with a sandwich ELISA kit (R&D Systems, Minneapolis, MN, USA; Catalog No. DCP00; sensitivity 10 pg/mL; Intra-CV = 7.8% and Inter-CV = 6.7%), and adiponectin concentration with an ELISA kit (Biovendor, Brno, Czech Republic; Catalog No. RD195023100; sensitivity 26 ng/mL; Intra-CV = 4.9% and Inter-CV = 6.7%). .. Furthermore, the lipid profile, anthropometric indices, and other markers were considered as secondary variables to adopt a structured approach in addressing multiplicity; in this regard, total cholesterol (TC) and triglycerides (TGs) were measured using enzymatic methods (CHOD-PAP), while HDL-C and LDL-C were measured via photometric methods (quantitative diagnostic kit by Pars Azmoon, Tehran, Iran; with a coefficient of 1.8% and sensitivity of 1 mg/dL for HDL, and a coefficient of 1.2% and sensitivity of 1 mg/dL for LDL), and leptin concentration was also determined with an ELISA kit (Biovendor, Czech Republic; Catalog No. RD191001100; sensitivity 0.2 ng/mL; Intra-CV = 5.9% and Inter-CV = 5.6%).

    Sandwich ELISA:

    Article Title: The Effects of Interval Resistance-Aerobic Training and Fisetin Supplementation on Asprosin and Selected Adipokines in Obese Men: A Double-Blind Randomized Control Trial.
    Article Snippet: .. Serum concentrations were analyzed using specific human enzyme-linked immunosorbent assay (ELISA) kits, among which Asprosin, MCP-1, and adiponectin were designated as primary outcomes; specifically, Asprosin concentration was measured with a sandwich ELISA kit (Elabscience Biotechnology, Wuhan, China; Catalog No. E-EL-H0515; detection range 0.156–10 ng/mL; sensitivity 0.094 ng/mL; Intra-assay CV < 10% and Inter-assay CV < 10%), MCP-1 concentration with a sandwich ELISA kit (R&D Systems, Minneapolis, MN, USA; Catalog No. DCP00; sensitivity 10 pg/mL; Intra-CV = 7.8% and Inter-CV = 6.7%), and adiponectin concentration with an ELISA kit (Biovendor, Brno, Czech Republic; Catalog No. RD195023100; sensitivity 26 ng/mL; Intra-CV = 4.9% and Inter-CV = 6.7%). .. Furthermore, the lipid profile, anthropometric indices, and other markers were considered as secondary variables to adopt a structured approach in addressing multiplicity; in this regard, total cholesterol (TC) and triglycerides (TGs) were measured using enzymatic methods (CHODPAP), while HDL-C and LDL-C were measured via photometric methods (quantitative diagnostic kit by Pars Azmoon, Tehran, Iran; with a coefficient of 1.8% and sensitivity of 1 mg/dL for HDL, and a coefficient of 1.2% and sensitivity of 1 mg/dL for LDL), and leptin concentration was also determined with an ELISA kit (Biovendor, Czech Republic; Catalog No. RD191001100; sensitivity 0.2 ng/mL; Intra-CV = 5.9% and Inter-CV = 5.6%).

    Article Title: Serum Perilipin-2 as a Novel Biomarker for Obstructive Sleep Apnea: Association with Hypoxic Burden and Disease Severity.
    Article Snippet: .. Assessment of Serum Perilipin-2 (PLIN 2) Concentration Serum PLIN-2 concentrations were determined using a commercially available sandwich ELISA kit (Human ADRP/Perilipin-2, Cat No: E-EL-H0278, Elabscience, Houston, TX, USA) according to the manufacturer’s instructions. ..

    Article Title: Therapeutic Potential of Beaucarnea recurvata Leaf Extract Against Ulcerative Colitis: Integrating Phytochemical Profiling, Network Pharmacology, and Experimental Validation
    Article Snippet: .. Epidermal growth factor receptor (EGFR) levels were measured using a sandwich ELISA kit (Elabscience E-EL-R0369, Houston, TX, USA), while SRC protein concentrations were determined via an ELISA assay (Abbexa abx547297, Cambridge, UK). .. Signal transducer and activator of transcription 3 (STAT3) was quantified using a rat-specific sandwich ELISA (MyBioSource MBS760293, San Diego, CA, USA), with a detection range of 0.156–10 ng/mL and a sensitivity of <0.094 ng/mL.

    Article Title: Hepatoprotective Activity of Red Ginger Extract on Acetaminophen-induced Liver Injury Models in HepG2 Cells.
    Article Snippet: Introduction: The liver is a major organ that plays a crucial role in detoxifying drugs; however, exposure to hepatotoxic drugs can lead to liver damage.. Some research indicates that Zingiber officinale, commonly known as red ginger, possesses various biological properties.. The objective of this study was to examine the hepatoprotective effect of red ginger extract (RGE) against acetaminophen (APAP)‐induced HepG2 cells.

    Article Title: The Effects of Interval Resistance—Aerobic Training and Fisetin Supplementation on Asprosin and Selected Adipokines in Obese Men: A Double-Blind Randomized Control Trial
    Article Snippet: .. Serum concentrations were analyzed using specific human enzyme-linked immunosorbent assay (ELISA) kits, among which Asprosin, MCP-1, and adiponectin were designated as primary outcomes; specifically, Asprosin concentration was measured with a sandwich ELISA kit (Elabscience Biotechnology, Wuhan, China; Catalog No. E-EL-H0515; detection range 0.156–10 ng/mL; sensitivity 0.094 ng/mL; Intra-assay CV < 10% and Inter-assay CV < 10%), MCP-1 concentration with a sandwich ELISA kit (R&D Systems, Minneapolis, MN, USA; Catalog No. DCP00; sensitivity 10 pg/mL; Intra-CV = 7.8% and Inter-CV = 6.7%), and adiponectin concentration with an ELISA kit (Biovendor, Brno, Czech Republic; Catalog No. RD195023100; sensitivity 26 ng/mL; Intra-CV = 4.9% and Inter-CV = 6.7%). .. Furthermore, the lipid profile, anthropometric indices, and other markers were considered as secondary variables to adopt a structured approach in addressing multiplicity; in this regard, total cholesterol (TC) and triglycerides (TGs) were measured using enzymatic methods (CHOD-PAP), while HDL-C and LDL-C were measured via photometric methods (quantitative diagnostic kit by Pars Azmoon, Tehran, Iran; with a coefficient of 1.8% and sensitivity of 1 mg/dL for HDL, and a coefficient of 1.2% and sensitivity of 1 mg/dL for LDL), and leptin concentration was also determined with an ELISA kit (Biovendor, Czech Republic; Catalog No. RD191001100; sensitivity 0.2 ng/mL; Intra-CV = 5.9% and Inter-CV = 5.6%).

    Article Title: The response of glutathione oxidase and cytochrome c to exercise training and sodium selenite in the heart tissue of male rats
    Article Snippet: Total protein concentrations were determined using the BCA assay kit (Thermo Fisher Scientific, USA). .. GPx activity was quantified using a commercial sandwich ELISA kit (Elabscience, Cat. No. E-EL-R2536, China) according to the manufacturer's instructions. ..

    Article Title: Serum Perilipin-2 as a Novel Biomarker for Obstructive Sleep Apnea: Association with Hypoxic Burden and Disease Severity
    Article Snippet: Standard biochemical markers were evaluated using an automated clinical chemistry analyzer (Siemens Dimension, Germany) in accordance with routine laboratory techniques. .. Serum PLIN-2 concentrations were determined using a commercially available sandwich ELISA kit (Human ADRP/Perilipin-2, Cat No: E-EL-H0278, Elabscience, Houston, TX, USA) according to the manufacturer’s instructions. ..

    Intra Assay:

    Article Title: The Effects of Interval Resistance-Aerobic Training and Fisetin Supplementation on Asprosin and Selected Adipokines in Obese Men: A Double-Blind Randomized Control Trial.
    Article Snippet: .. Serum concentrations were analyzed using specific human enzyme-linked immunosorbent assay (ELISA) kits, among which Asprosin, MCP-1, and adiponectin were designated as primary outcomes; specifically, Asprosin concentration was measured with a sandwich ELISA kit (Elabscience Biotechnology, Wuhan, China; Catalog No. E-EL-H0515; detection range 0.156–10 ng/mL; sensitivity 0.094 ng/mL; Intra-assay CV < 10% and Inter-assay CV < 10%), MCP-1 concentration with a sandwich ELISA kit (R&D Systems, Minneapolis, MN, USA; Catalog No. DCP00; sensitivity 10 pg/mL; Intra-CV = 7.8% and Inter-CV = 6.7%), and adiponectin concentration with an ELISA kit (Biovendor, Brno, Czech Republic; Catalog No. RD195023100; sensitivity 26 ng/mL; Intra-CV = 4.9% and Inter-CV = 6.7%). .. Furthermore, the lipid profile, anthropometric indices, and other markers were considered as secondary variables to adopt a structured approach in addressing multiplicity; in this regard, total cholesterol (TC) and triglycerides (TGs) were measured using enzymatic methods (CHODPAP), while HDL-C and LDL-C were measured via photometric methods (quantitative diagnostic kit by Pars Azmoon, Tehran, Iran; with a coefficient of 1.8% and sensitivity of 1 mg/dL for HDL, and a coefficient of 1.2% and sensitivity of 1 mg/dL for LDL), and leptin concentration was also determined with an ELISA kit (Biovendor, Czech Republic; Catalog No. RD191001100; sensitivity 0.2 ng/mL; Intra-CV = 5.9% and Inter-CV = 5.6%).

    Article Title: The Effects of Interval Resistance—Aerobic Training and Fisetin Supplementation on Asprosin and Selected Adipokines in Obese Men: A Double-Blind Randomized Control Trial
    Article Snippet: .. Serum concentrations were analyzed using specific human enzyme-linked immunosorbent assay (ELISA) kits, among which Asprosin, MCP-1, and adiponectin were designated as primary outcomes; specifically, Asprosin concentration was measured with a sandwich ELISA kit (Elabscience Biotechnology, Wuhan, China; Catalog No. E-EL-H0515; detection range 0.156–10 ng/mL; sensitivity 0.094 ng/mL; Intra-assay CV < 10% and Inter-assay CV < 10%), MCP-1 concentration with a sandwich ELISA kit (R&D Systems, Minneapolis, MN, USA; Catalog No. DCP00; sensitivity 10 pg/mL; Intra-CV = 7.8% and Inter-CV = 6.7%), and adiponectin concentration with an ELISA kit (Biovendor, Brno, Czech Republic; Catalog No. RD195023100; sensitivity 26 ng/mL; Intra-CV = 4.9% and Inter-CV = 6.7%). .. Furthermore, the lipid profile, anthropometric indices, and other markers were considered as secondary variables to adopt a structured approach in addressing multiplicity; in this regard, total cholesterol (TC) and triglycerides (TGs) were measured using enzymatic methods (CHOD-PAP), while HDL-C and LDL-C were measured via photometric methods (quantitative diagnostic kit by Pars Azmoon, Tehran, Iran; with a coefficient of 1.8% and sensitivity of 1 mg/dL for HDL, and a coefficient of 1.2% and sensitivity of 1 mg/dL for LDL), and leptin concentration was also determined with an ELISA kit (Biovendor, Czech Republic; Catalog No. RD191001100; sensitivity 0.2 ng/mL; Intra-CV = 5.9% and Inter-CV = 5.6%).

    Inter Assay:

    Article Title: The Effects of Interval Resistance-Aerobic Training and Fisetin Supplementation on Asprosin and Selected Adipokines in Obese Men: A Double-Blind Randomized Control Trial.
    Article Snippet: .. Serum concentrations were analyzed using specific human enzyme-linked immunosorbent assay (ELISA) kits, among which Asprosin, MCP-1, and adiponectin were designated as primary outcomes; specifically, Asprosin concentration was measured with a sandwich ELISA kit (Elabscience Biotechnology, Wuhan, China; Catalog No. E-EL-H0515; detection range 0.156–10 ng/mL; sensitivity 0.094 ng/mL; Intra-assay CV < 10% and Inter-assay CV < 10%), MCP-1 concentration with a sandwich ELISA kit (R&D Systems, Minneapolis, MN, USA; Catalog No. DCP00; sensitivity 10 pg/mL; Intra-CV = 7.8% and Inter-CV = 6.7%), and adiponectin concentration with an ELISA kit (Biovendor, Brno, Czech Republic; Catalog No. RD195023100; sensitivity 26 ng/mL; Intra-CV = 4.9% and Inter-CV = 6.7%). .. Furthermore, the lipid profile, anthropometric indices, and other markers were considered as secondary variables to adopt a structured approach in addressing multiplicity; in this regard, total cholesterol (TC) and triglycerides (TGs) were measured using enzymatic methods (CHODPAP), while HDL-C and LDL-C were measured via photometric methods (quantitative diagnostic kit by Pars Azmoon, Tehran, Iran; with a coefficient of 1.8% and sensitivity of 1 mg/dL for HDL, and a coefficient of 1.2% and sensitivity of 1 mg/dL for LDL), and leptin concentration was also determined with an ELISA kit (Biovendor, Czech Republic; Catalog No. RD191001100; sensitivity 0.2 ng/mL; Intra-CV = 5.9% and Inter-CV = 5.6%).

    Article Title: The Effects of Interval Resistance—Aerobic Training and Fisetin Supplementation on Asprosin and Selected Adipokines in Obese Men: A Double-Blind Randomized Control Trial
    Article Snippet: .. Serum concentrations were analyzed using specific human enzyme-linked immunosorbent assay (ELISA) kits, among which Asprosin, MCP-1, and adiponectin were designated as primary outcomes; specifically, Asprosin concentration was measured with a sandwich ELISA kit (Elabscience Biotechnology, Wuhan, China; Catalog No. E-EL-H0515; detection range 0.156–10 ng/mL; sensitivity 0.094 ng/mL; Intra-assay CV < 10% and Inter-assay CV < 10%), MCP-1 concentration with a sandwich ELISA kit (R&D Systems, Minneapolis, MN, USA; Catalog No. DCP00; sensitivity 10 pg/mL; Intra-CV = 7.8% and Inter-CV = 6.7%), and adiponectin concentration with an ELISA kit (Biovendor, Brno, Czech Republic; Catalog No. RD195023100; sensitivity 26 ng/mL; Intra-CV = 4.9% and Inter-CV = 6.7%). .. Furthermore, the lipid profile, anthropometric indices, and other markers were considered as secondary variables to adopt a structured approach in addressing multiplicity; in this regard, total cholesterol (TC) and triglycerides (TGs) were measured using enzymatic methods (CHOD-PAP), while HDL-C and LDL-C were measured via photometric methods (quantitative diagnostic kit by Pars Azmoon, Tehran, Iran; with a coefficient of 1.8% and sensitivity of 1 mg/dL for HDL, and a coefficient of 1.2% and sensitivity of 1 mg/dL for LDL), and leptin concentration was also determined with an ELISA kit (Biovendor, Czech Republic; Catalog No. RD191001100; sensitivity 0.2 ng/mL; Intra-CV = 5.9% and Inter-CV = 5.6%).

    Activity Assay:

    Article Title: The response of glutathione oxidase and cytochrome c to exercise training and sodium selenite in the heart tissue of male rats
    Article Snippet: Total protein concentrations were determined using the BCA assay kit (Thermo Fisher Scientific, USA). .. GPx activity was quantified using a commercial sandwich ELISA kit (Elabscience, Cat. No. E-EL-R2536, China) according to the manufacturer's instructions. ..



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    AMBP promotes imbalance of BCAA metabolism. (A) mRNA transcripts of AMBP in patients with different grades of IVDD and relative quantitative analysis. (B) mRNA transcripts of AMBP in TNF-α-stimulated HNPC cells and relative quantitative analysis. (C) Detection of inhibition efficiency of small interfering RNAs against different targets. (D) Serum concentrations of AMBP in normal, T2DM, IVDD, and T2DM combined with IVDD patients were examined using <t>ELISA.</t> (E) Representative β-gal staining images of HNPC cells before and after AMBP inhibition. (F) Correlation analysis between AMBP expression and β-gal expression. (G) mRNA transcripts of BCAA metabolism-related enzymes in TNF-α-stimulated HNPC cells before and after AMBP inhibition and relative quantitative analysis.
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    Club cell AHR is required for EPFR-induced pulmonary Th17 responses and neutrophilic inflammation. (a) Schematic of the experimental design: tamoxifen was administered for 5 consecutive days to induce Cre recombinase-mediated deletion of Ahr in club cells, followed by a 7-day washout period. Male and female Cre-negative Ahr fl/fl LM or Ahr ΔCC mice were then exposed to air or EPFRs by inhalation from day 0 through day 6. Lungs and bronchoalveolar lavage fluid (BALF) were collected on day 6 post-exposure. (b) Flow cytometric quantification of Th17 cells i.e. IL-17 producing CD4 + T cells (Lin − CD45 + CD3 + CD4 + ) in lungs of air or EPFR-exposed LM and Ahr ΔCC mice; n = 6 to 10 mice per group. Data are presented as mean ± SEM and normalized to air controls of the respective genotype. Statistical significance was determined using Student's t-test (∗p < 0.05) (c) Concatenated pseudo color dot plots of CD4 + T cells gated <t>for</t> <t>IL-17A</t> in air or EPFR-exposed LM and Ahr ΔCC mice; n = 6 to 10 mice. Data are presented as mean ± SEM and normalized to air controls of the respective genotype. Statistical significance was determined using Student's t-test (∗p < 0.05) (d) Total and differential BALF cell counts from air or EPFR-exposed LM and Ahr ΔCC mice; n = 4 to 6 mice per group. Data are presented as mean ± SEM. Statistical significance was determined using one-way ANOVA followed by Tukey's post-hoc test; a p <0.05 compared to Air-LM and b p < 0.05 compared to EPFR-LM. (e) Representative BALF cell images from LM and Ahr ΔCC mice exposed to air or EPFR; arrows indicate neutrophils. Scale bar = 25 μm. (f-j) Analysis of BALF pro-inflammatory cytokines (IL-17, KC/GRO, IL-6, IL-1β, TNF-α) from air or EPFR-exposed LM and Ahr ΔCC mice; n = 4 to 7 mice per group. Data are presented as mean ± SEM and normalized to air controls of the respective genotype. Statistical significance was determined using Student's t-test (∗p < 0.05).
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    Club cell AHR is required for EPFR-induced pulmonary Th17 responses and neutrophilic inflammation. (a) Schematic of the experimental design: tamoxifen was administered for 5 consecutive days to induce Cre recombinase-mediated deletion of Ahr in club cells, followed by a 7-day washout period. Male and female Cre-negative Ahr fl/fl LM or Ahr ΔCC mice were then exposed to air or EPFRs by inhalation from day 0 through day 6. Lungs and bronchoalveolar lavage fluid (BALF) were collected on day 6 post-exposure. (b) Flow cytometric quantification of Th17 cells i.e. IL-17 producing CD4 + T cells (Lin − CD45 + CD3 + CD4 + ) in lungs of air or EPFR-exposed LM and Ahr ΔCC mice; n = 6 to 10 mice per group. Data are presented as mean ± SEM and normalized to air controls of the respective genotype. Statistical significance was determined using Student's t-test (∗p < 0.05) (c) Concatenated pseudo color dot plots of CD4 + T cells gated <t>for</t> <t>IL-17A</t> in air or EPFR-exposed LM and Ahr ΔCC mice; n = 6 to 10 mice. Data are presented as mean ± SEM and normalized to air controls of the respective genotype. Statistical significance was determined using Student's t-test (∗p < 0.05) (d) Total and differential BALF cell counts from air or EPFR-exposed LM and Ahr ΔCC mice; n = 4 to 6 mice per group. Data are presented as mean ± SEM. Statistical significance was determined using one-way ANOVA followed by Tukey's post-hoc test; a p <0.05 compared to Air-LM and b p < 0.05 compared to EPFR-LM. (e) Representative BALF cell images from LM and Ahr ΔCC mice exposed to air or EPFR; arrows indicate neutrophils. Scale bar = 25 μm. (f-j) Analysis of BALF pro-inflammatory cytokines (IL-17, KC/GRO, IL-6, IL-1β, TNF-α) from air or EPFR-exposed LM and Ahr ΔCC mice; n = 4 to 7 mice per group. Data are presented as mean ± SEM and normalized to air controls of the respective genotype. Statistical significance was determined using Student's t-test (∗p < 0.05).
    Mouse Troponin I Type 3 Cardiac Sandwich Elisa Kit, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    sandwich enzyme linked immunosorbent assay kit  (Kainos Laboratories)
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    Club cell AHR is required for EPFR-induced pulmonary Th17 responses and neutrophilic inflammation. (a) Schematic of the experimental design: tamoxifen was administered for 5 consecutive days to induce Cre recombinase-mediated deletion of Ahr in club cells, followed by a 7-day washout period. Male and female Cre-negative Ahr fl/fl LM or Ahr ΔCC mice were then exposed to air or EPFRs by inhalation from day 0 through day 6. Lungs and bronchoalveolar lavage fluid (BALF) were collected on day 6 post-exposure. (b) Flow cytometric quantification of Th17 cells i.e. IL-17 producing CD4 + T cells (Lin − CD45 + CD3 + CD4 + ) in lungs of air or EPFR-exposed LM and Ahr ΔCC mice; n = 6 to 10 mice per group. Data are presented as mean ± SEM and normalized to air controls of the respective genotype. Statistical significance was determined using Student's t-test (∗p < 0.05) (c) Concatenated pseudo color dot plots of CD4 + T cells gated <t>for</t> <t>IL-17A</t> in air or EPFR-exposed LM and Ahr ΔCC mice; n = 6 to 10 mice. Data are presented as mean ± SEM and normalized to air controls of the respective genotype. Statistical significance was determined using Student's t-test (∗p < 0.05) (d) Total and differential BALF cell counts from air or EPFR-exposed LM and Ahr ΔCC mice; n = 4 to 6 mice per group. Data are presented as mean ± SEM. Statistical significance was determined using one-way ANOVA followed by Tukey's post-hoc test; a p <0.05 compared to Air-LM and b p < 0.05 compared to EPFR-LM. (e) Representative BALF cell images from LM and Ahr ΔCC mice exposed to air or EPFR; arrows indicate neutrophils. Scale bar = 25 μm. (f-j) Analysis of BALF pro-inflammatory cytokines (IL-17, KC/GRO, IL-6, IL-1β, TNF-α) from air or EPFR-exposed LM and Ahr ΔCC mice; n = 4 to 7 mice per group. Data are presented as mean ± SEM and normalized to air controls of the respective genotype. Statistical significance was determined using Student's t-test (∗p < 0.05).
    Sandwich Enzyme Linked Immunosorbent Assay Kit, supplied by Kainos Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Club cell AHR is required for EPFR-induced pulmonary Th17 responses and neutrophilic inflammation. (a) Schematic of the experimental design: tamoxifen was administered for 5 consecutive days to induce Cre recombinase-mediated deletion of Ahr in club cells, followed by a 7-day washout period. Male and female Cre-negative Ahr fl/fl LM or Ahr ΔCC mice were then exposed to air or EPFRs by inhalation from day 0 through day 6. Lungs and bronchoalveolar lavage fluid (BALF) were collected on day 6 post-exposure. (b) Flow cytometric quantification of Th17 cells i.e. IL-17 producing CD4 + T cells (Lin − CD45 + CD3 + CD4 + ) in lungs of air or EPFR-exposed LM and Ahr ΔCC mice; n = 6 to 10 mice per group. Data are presented as mean ± SEM and normalized to air controls of the respective genotype. Statistical significance was determined using Student's t-test (∗p < 0.05) (c) Concatenated pseudo color dot plots of CD4 + T cells gated <t>for</t> <t>IL-17A</t> in air or EPFR-exposed LM and Ahr ΔCC mice; n = 6 to 10 mice. Data are presented as mean ± SEM and normalized to air controls of the respective genotype. Statistical significance was determined using Student's t-test (∗p < 0.05) (d) Total and differential BALF cell counts from air or EPFR-exposed LM and Ahr ΔCC mice; n = 4 to 6 mice per group. Data are presented as mean ± SEM. Statistical significance was determined using one-way ANOVA followed by Tukey's post-hoc test; a p <0.05 compared to Air-LM and b p < 0.05 compared to EPFR-LM. (e) Representative BALF cell images from LM and Ahr ΔCC mice exposed to air or EPFR; arrows indicate neutrophils. Scale bar = 25 μm. (f-j) Analysis of BALF pro-inflammatory cytokines (IL-17, KC/GRO, IL-6, IL-1β, TNF-α) from air or EPFR-exposed LM and Ahr ΔCC mice; n = 4 to 7 mice per group. Data are presented as mean ± SEM and normalized to air controls of the respective genotype. Statistical significance was determined using Student's t-test (∗p < 0.05).
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    Image Search Results


    AMBP promotes imbalance of BCAA metabolism. (A) mRNA transcripts of AMBP in patients with different grades of IVDD and relative quantitative analysis. (B) mRNA transcripts of AMBP in TNF-α-stimulated HNPC cells and relative quantitative analysis. (C) Detection of inhibition efficiency of small interfering RNAs against different targets. (D) Serum concentrations of AMBP in normal, T2DM, IVDD, and T2DM combined with IVDD patients were examined using ELISA. (E) Representative β-gal staining images of HNPC cells before and after AMBP inhibition. (F) Correlation analysis between AMBP expression and β-gal expression. (G) mRNA transcripts of BCAA metabolism-related enzymes in TNF-α-stimulated HNPC cells before and after AMBP inhibition and relative quantitative analysis.

    Journal: Biochemistry and Biophysics Reports

    Article Title: Screening of metabolic-related biomarkers linking intervertebral disc degeneration and type 2 diabetes based on comprehensive bioinformatics analysis and machine learning

    doi: 10.1016/j.bbrep.2026.102593

    Figure Lengend Snippet: AMBP promotes imbalance of BCAA metabolism. (A) mRNA transcripts of AMBP in patients with different grades of IVDD and relative quantitative analysis. (B) mRNA transcripts of AMBP in TNF-α-stimulated HNPC cells and relative quantitative analysis. (C) Detection of inhibition efficiency of small interfering RNAs against different targets. (D) Serum concentrations of AMBP in normal, T2DM, IVDD, and T2DM combined with IVDD patients were examined using ELISA. (E) Representative β-gal staining images of HNPC cells before and after AMBP inhibition. (F) Correlation analysis between AMBP expression and β-gal expression. (G) mRNA transcripts of BCAA metabolism-related enzymes in TNF-α-stimulated HNPC cells before and after AMBP inhibition and relative quantitative analysis.

    Article Snippet: Cytokine quantification was performed using a commercial double-antibody sandwich ELISA kit (Elabscience, E-EL-H0315, AMBP) according to the manufacturer's instructions.

    Techniques: Inhibition, Enzyme-linked Immunosorbent Assay, Staining, Expressing

    Club cell AHR is required for EPFR-induced pulmonary Th17 responses and neutrophilic inflammation. (a) Schematic of the experimental design: tamoxifen was administered for 5 consecutive days to induce Cre recombinase-mediated deletion of Ahr in club cells, followed by a 7-day washout period. Male and female Cre-negative Ahr fl/fl LM or Ahr ΔCC mice were then exposed to air or EPFRs by inhalation from day 0 through day 6. Lungs and bronchoalveolar lavage fluid (BALF) were collected on day 6 post-exposure. (b) Flow cytometric quantification of Th17 cells i.e. IL-17 producing CD4 + T cells (Lin − CD45 + CD3 + CD4 + ) in lungs of air or EPFR-exposed LM and Ahr ΔCC mice; n = 6 to 10 mice per group. Data are presented as mean ± SEM and normalized to air controls of the respective genotype. Statistical significance was determined using Student's t-test (∗p < 0.05) (c) Concatenated pseudo color dot plots of CD4 + T cells gated for IL-17A in air or EPFR-exposed LM and Ahr ΔCC mice; n = 6 to 10 mice. Data are presented as mean ± SEM and normalized to air controls of the respective genotype. Statistical significance was determined using Student's t-test (∗p < 0.05) (d) Total and differential BALF cell counts from air or EPFR-exposed LM and Ahr ΔCC mice; n = 4 to 6 mice per group. Data are presented as mean ± SEM. Statistical significance was determined using one-way ANOVA followed by Tukey's post-hoc test; a p <0.05 compared to Air-LM and b p < 0.05 compared to EPFR-LM. (e) Representative BALF cell images from LM and Ahr ΔCC mice exposed to air or EPFR; arrows indicate neutrophils. Scale bar = 25 μm. (f-j) Analysis of BALF pro-inflammatory cytokines (IL-17, KC/GRO, IL-6, IL-1β, TNF-α) from air or EPFR-exposed LM and Ahr ΔCC mice; n = 4 to 7 mice per group. Data are presented as mean ± SEM and normalized to air controls of the respective genotype. Statistical significance was determined using Student's t-test (∗p < 0.05).

    Journal: Redox Biology

    Article Title: Aryl hydrocarbon receptor in club cells drives Th17-mediated lung injury following inhalation exposure to environmentally persistent free radicals

    doi: 10.1016/j.redox.2026.104105

    Figure Lengend Snippet: Club cell AHR is required for EPFR-induced pulmonary Th17 responses and neutrophilic inflammation. (a) Schematic of the experimental design: tamoxifen was administered for 5 consecutive days to induce Cre recombinase-mediated deletion of Ahr in club cells, followed by a 7-day washout period. Male and female Cre-negative Ahr fl/fl LM or Ahr ΔCC mice were then exposed to air or EPFRs by inhalation from day 0 through day 6. Lungs and bronchoalveolar lavage fluid (BALF) were collected on day 6 post-exposure. (b) Flow cytometric quantification of Th17 cells i.e. IL-17 producing CD4 + T cells (Lin − CD45 + CD3 + CD4 + ) in lungs of air or EPFR-exposed LM and Ahr ΔCC mice; n = 6 to 10 mice per group. Data are presented as mean ± SEM and normalized to air controls of the respective genotype. Statistical significance was determined using Student's t-test (∗p < 0.05) (c) Concatenated pseudo color dot plots of CD4 + T cells gated for IL-17A in air or EPFR-exposed LM and Ahr ΔCC mice; n = 6 to 10 mice. Data are presented as mean ± SEM and normalized to air controls of the respective genotype. Statistical significance was determined using Student's t-test (∗p < 0.05) (d) Total and differential BALF cell counts from air or EPFR-exposed LM and Ahr ΔCC mice; n = 4 to 6 mice per group. Data are presented as mean ± SEM. Statistical significance was determined using one-way ANOVA followed by Tukey's post-hoc test; a p <0.05 compared to Air-LM and b p < 0.05 compared to EPFR-LM. (e) Representative BALF cell images from LM and Ahr ΔCC mice exposed to air or EPFR; arrows indicate neutrophils. Scale bar = 25 μm. (f-j) Analysis of BALF pro-inflammatory cytokines (IL-17, KC/GRO, IL-6, IL-1β, TNF-α) from air or EPFR-exposed LM and Ahr ΔCC mice; n = 4 to 7 mice per group. Data are presented as mean ± SEM and normalized to air controls of the respective genotype. Statistical significance was determined using Student's t-test (∗p < 0.05).

    Article Snippet: IL-17A levels in BALF samples were measured using a commercially available Mouse IL-17A Sandwich ELISA Kit (Proteintech, Rosemont, IL; catalog# KE10020) according to the manufacturer's instructions.

    Techniques: